Caged RNA: photo-control of a ribozyme reaction

نویسندگان

  • Steven G. Chaulk
  • Andrew M. MacMillan
چکیده

We report here the first photo-chemical control of a ribozyme reaction by the site-specific modification of the 2'-hydroxyl nucleophile in the hammerhead system with a caging functionality. Rapid laser photolysis of the O-(2-nitrobenzyl) caging group initiates an efficient and accurate hammerhead-catalyzed cleavage of substrate RNA under native conditions. RNAs in which reactive functionalities or recognition elements are caged in this manner will be useful tools to probe RNA reactivity and dynamics.

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عنوان ژورنال:
  • Nucleic acids research

دوره 26 13  شماره 

صفحات  -

تاریخ انتشار 1998